Prevalence, serotypes distribution and characterization of Salmonella in common carp (Cyprinus Carpio), Afyonkarahisar Province, Turkey

Abstract

Abstract<br /> <br /> A total 100 free fresh water common carp were screened for the prevalence of Salmonella spp., to determine the invA gene, serotyping, antibiotic resistance profile and the presence of class 1 integron (Cls1) and integrase (intI1) genes of the isolates. To detect Salmonella, the classic culture technique; and to detect the presence of invA, Cls1 and intI1, the PCR assay were applied. Salmonella were detected in 3% (n=7 isolates). Three different serotypes were detected; S. Panama, Enteritidis and Quinella. S. Panama is predominant. The invA was detected in the isolates. To detect antibiotic susceptibility, 14 different antibiotics were evaluated using the disc diffusion method. The isolates were evaluated for β-lactamase production. All isolates were resistant to erythromycin, penicillin, oxacillin, vancomycin and clindamycin. Multiple resistances were found in all isolates. S. Enteritidis and one S. Panama were the most resistant serovars. The Cls1 were detected in all isolates. In contrast, int1 were detected in 57.14%. In conclusion, the prevalence of Salmonella in the common carp can pose a risk to the public due to causing foodborne salmonellosis, multiple antibiotic resistance properties and potential transfer of drug resistance genes to other members of the Enterobacteriaceae and humans via Cls1 integron.