Micropropagation of Clematis orientalis L. culture in vitro

Abstract

Background & Aim:<em> Clematis </em>is an ornamental and medicinal plant that is primarily planted as an ornamental plant. <em>Clematis orientalis</em> is native to Asia and Central Europe, used as an antiseptic and refrigerant. The propagation of <em>Clematis</em> is very important. In this study, the effects of plant growth regulators on rooting and growth of the plants <em>in vitro</em> culture were examined. Experimental: In this study, effects of growth regulators, including naphthalene acetic acid (NAA), at the concentrations of 0.0, 0.4, 0.5, and 0.6 mg/mL and Indol butyric acid (IBA) at the concentrations of 0.0, 0.4, 0.5, and 0.6 mg/mL on micropropagationin Murashige and Skoog's medium (MS) were investigated. Results & Discussion: Results indicated that the effects growth regulators of auxin at different concentrations on in vitro growth micrpropagules were significant. The highest rooting and number of stems were obtained from control. Increase concentration of growth regulators decreased rooting. The highest decrease rate was obtained from0.6 mg/L auxin. Recommended applications/industries: The result of study indicated that positive effects lateral shoot explants and Murashige and Skoog's medium (MS) without growth regulators on micropropagation.