The Effect of Spinal and General Anesthesia on Serum Lipid Peroxides and Total Antioxidant Capacity in Diabetic Patients with Lower Limb Amputation Surgery

Abstract

Background: Anesthesia is performed in two major methods including regional and general.<br />The aim of this study was to compare the effect of anesthesia method (spinal and general) on oxidative stress in<br />diabetic patients underwent diabetic amputation surgery.<br />Methods: In this randomized control trial, 40 patients with diabetic foot who were candidate for foot amputation<br />surgery at our academic hospital in 2013, were selected and divided into two groups based on anesthesia method.<br />Lipid peroxide level and serum total antioxidant capacity (TAC) were measured before anesthesia induction and one<br />hour after surgery. As the normal range, the findings obtained from 23 healthy volunteers were utilized.<br />Results: Mean age was 54.9±11.21 and 52.4±11.23 years in the spinal anesthesia (SA) and the general anesthesia<br />(GA) group, respectively (P=0.49). Serum TAC in GA group increased from 1.03±0.04 mM to 2.98±0.7 mM. In SA<br />group, the increase of serum TAC from 1.22±0.11 mM to 3.42±0.5 mM was observed that indicated the increase of<br />serum TAC in both groups was not significantly different (P=0.21). Serum Malondialdehyde (MDA) in GA and SA<br />groups did not show a significant difference before surgery (31.14±3.9 mM vs. 29.06±2.49 mM in GA and SA groups,<br />respectively) (P=0.31), while it was significantly different after surgery (23.14±2.6 mM and 19.24±2.7 mM in GA and SA<br />groups, respectively) (P=0.03).<br />Conclusion: lower limb amputation can help to control oxidative stress in diabetic patients; and considering serum<br />MDA as a marker of oxidative stress, SA seems to be more effective to control this problem.