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    • Archives of Razi Institute
    • Volume 71, Issue 2
    • مشاهده مورد
    •   صفحهٔ اصلی
    • نشریات انگلیسی
    • Archives of Razi Institute
    • Volume 71, Issue 2
    • مشاهده مورد
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    S1 gene sequence analysis of infectious bronchitis virus vaccinal strains (H120 & H52) and their embryo-passaged derivatives

    (ندگان)پدیدآور
    Bakhshesh, M.Masoudi, S.Esmailizad, M.Khalesi, B.
    Thumbnail
    نوع مدرک
    Text
    Original Articles
    زبان مدرک
    English
    نمایش کامل رکورد
    چکیده
    Avian infectious bronchitis is an acute and highly contagious disease that mainly causes respiratory symptoms in poultry. A number of serotypes and variants of the viral agent with poor cross-protection are the major problem to achieve desired immunity from vaccination. The S1 subunit of S glycoprotein (spike) is the major determinant of IBV so that a minor change in amino acid sequence of this protein, alters the virus strain. Therefore, characterization of the sequence of S1 gene is necessary to identify virus strains and their similarities with the vaccinal strains. In this research, the S1 sequence of H52 and H120 vaccinal strains of Razi Institute was fully characterized, and also the effect of serial passages in embryonated - eggs (5 passages beyond the master seed) on the S1 gene was investigated. The results showed that H120 and H52 strains of Razi Institute are 100% identical to the reference vaccine strains available in the GenBank. In addition, the H52 strain showed one amino acid substitution from the 3rd passage in which Glycine (G) was replaced by Valine (V) at position 118 making these passages exactly identical to the H120 strain while no change occurred for the H120 strain during these passages. Analysis of the original vaccinal strains which are widely administered in Iran, is definitely useful for prevention and control strategies against the circulating viruses. To identify the genetic change(s) responsible for attenuation of these strains during passages in embryonated-egg, characterization of other genes, especially those involved in replication is recommended.
    کلید واژگان
    IBV
    Vaccinal strain
    S1 gene characterization
    egg- passaged
    Veterinary Virology

    شماره نشریه
    2
    تاریخ نشر
    2016-06-01
    1395-03-12
    ناشر
    Razi Vaccine & Serum Research Institute
    سازمان پدید آورنده
    Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
    Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
    Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
    Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

    شاپا
    0365-3439
    2008-9872
    URI
    https://dx.doi.org/10.22034/ari.2016.106446
    https://archrazi.areeo.ac.ir/article_106446.html
    https://iranjournals.nlai.ir/handle/123456789/419602

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