Genetic Variation Among Salvia Species Based on Sequence-Related Amplified Polymorphism (SRAP) Marker

Abstract

In this study, SRAP molecular maker approach was performed to investigate genetic diversity in the <em>Salvia</em> genus. A total of 205 DNA bands were produced from PCR amplification of 11 <em>Salvia</em> species and populations using 25 selective primer combinations, of which 204 polymorphic genetic loci accounted. The total number of amplified fragments ranged from 3 to 15. The genetic similarities of 11 collections were estimated from 0.0968 to 0.6949. According to the SRAP marker analysis, <em>S.verticillata2</em> and <em>S.nemorosa2</em> had the lowest similarityand the species of<em> S.verticillata2</em> and <em>S.verticillata3</em> had the highest similarity. Unweighted pair-group method with arithmetic averages clustering algorithm categorized <em>Salvia </em>species into six major groups. In general, SRAP markers were able to detect high polymorphism among <em>Salvia</em> genotypes. Hence, the technique is regarded as a reliable tool for differentiation of <em>Salvia </em>at inter and intra-species level.