Amino acid and fatty acid profiles of materials recovered from Prussian carp, Carassius gibelio (Bloch, 1782), using acidic and basic solubilization/ precipitation technique

Abstract

Isoelectric solubilization /precipitation (ISP) process was used to isolate protein from muscles of Prussian carp, Carassius gibelio (Bloch, 1782). Fish protein and lipid were recovered from whole gutted Prussian carp using acidic and basic isoelectric solubilization/precipitation followed by assaying amino acid and fatty acid profile. Essential amino acids content in acidic and basic pH treatment of ISP of Prussian carp were 216.6 and 218.7 mg.g-1, respectively. Results showed that identified amino acids in Prussian carp protein isolated by ISP method, could meet all needs of adults, but a supplementary protein must be used for children. Limiting amino acids in both acidic and basic treatments were methionine and cysteine. Nineteen fatty acids of saturated (SFA), monounsaturated (MUFA) and polyunsaturated (PUFA) groups were identified in lipid recovered in isoelectric solubilization/precipitation process. Total PUFA in basic treatment was noticeably higher than in acidic one. N-3/n-6 ratio in basic pH treatment was also higher than in acidic one.