Comparison of Cellular Toxicity of Iranian and Non-Iranian Self Cure Acryl and their Effects on Production of Mouse IL-6 by L929 Gingival Fibroblasts

Abstract

Statement of problem: Despite the various methods used to initiate the polymerization of resin, the conversion of monomers to polymers is not complete and some unreacted monomers are left in the polymer that is leachable into saliva. Substance leached from resin can cause adverse reactions in the oral mucosa adjacent to the denture. Aim: The aim of this study was the comparison of cellular toxicity of Iranian and non Iranian self cure acryl on mouse L929 gingival fibroblasts.Materials and method: Three types of acryl including Trim acryl (poly vinyl Ethyl Methacrylate), Duralay acryl (poly methyl Methacrylate) and Acropars acryl (poly methyl methacrylate) were used. A total of 90 rings (30 for each type) with predetermined sizes were prepared. Suspension of fibroblasts with acrylic rings was put in 24-wells culture plates and a culture media containing RPMI-1640 environment plus antibiotics and 10% FBS were added. The plates were incubated in the Co2 incubator and after one hour, 24 hours and seven days they were checked for cytotoxicity by MTT test and secretion of IL-6 by ELISA technique. Two-way ANOVA, Kruskal-wallis and Mann-Whitney U tests were used for data analysis.Results: Significant differences were found in the biocompatibility of experimental acryl, Trim and Duralay (p<0.05) Acropars and Duralay (p<0.01) and Acropars and Trim (p<0.001), among studied time intervals. No statistically significant difference was seen in the production of IL-6 among these acryl (p=0.120). Least cytotoxicity was seen at interval of 24 hours with significant statistically difference between one hour and one week time intervals (p<0.001).Conclusion: This study showed that the acryl having methyl methacrylate (Acropars and duralay) have greater toxic effects on fibroblasts; although the toxic effects of Acropars were more than Duralay. The least cytotoxicity was seen in Trim which has vinylethyl methacrylate. There was no difference among studied acryl on induction of IL-6.Key words: Cellular toxicity, Self- cure acryl, IL-6 production, Gingival fibroblasts (L-929)