Association Between the c.3751G>A Genetic Variant of MDR1 and Hepatocellular Carcinoma Risk in a Chinese Han Population

Abstract

The objective of this study was to evaluate the influence of a genetic variant in the multidrug resistance 1 gene(MDR1) on hepatocellular carcinoma (HCC) risk. This case-control study was conducted in a Chinese populationof 645 HCC cases and 658 cancer-free controls. The genotype of the c.3751G>A genetic variant in the MDR1 genewas investigated by created restriction site-polymerase chain reaction (CRS-PCR) and DNA sequencing methods.Our data demonstrated significantly differences detected in the allelic and genotypic frequencies between HCCcases and those of cancer-free controls. Association analyses indicated that there were statistically increasedrisk of HCC in the homozygote comparison (AA versus (vs.) GG: OR = 2.22, 95% CI 1.51-3.27, χ2 = 16.90, P< 0.001), dominant model (AA/GA vs. GG: OR = 1.25, 95% CI 1.00-1.55, χ2 = 3.98, P = 0.046), recessive model(AA vs. GA/GG: OR = 2.14, 95% CI 1.47-3.09, χ2 = 16.68, P < 0.001) and allele comparison (A vs. G: OR = 1.33,95% CI 1.13-1.57, χ2 = 11.66, P = 0.001). The allele-A and genotype-AA may contribute to HCC susceptibility.These preliminary findings suggest that the c.3751G>A genetic variant in the MDR1 gene is potentially relatedto HCC susceptibility in a Chinese Han population, and might be used as a molecular marker for evaluatingHCC susceptibility.