Expression and Clinical Significance of Osteopontin in Calcified Breast Tissue

Abstract

Osteopontin (OPN) is an integrin-binding protein, believed to be involved in a variety of physiological cellularfunctions. The physiology of OPN is best documented in the bone where this secreted adhesive glycoprotein appearsto be involved in osteoblast differentiation and bone formation. In our study, we used semi-quantitative RT-PCRof osteopontin in calcification tissue of breast to detect breast cancer metastasis. The obtained data indicate thatthe expression of osteopontin is related to calcification tissue of breast, and possibly with the incidence of breastcancer. The expression strength of OPN by RT-PCR detection was related to the degree of malignancy of breastlesions, suggesting a close relationship between OPN and breast calcification tissue. The results revealed thatexpression of OPN mRNA is related to calcification of breast cancer tissue and to the development of breastcancer. Determination of OPN mRNA expression can be expected to be a guide to clinical therapy and predictionof the prognosis of breast cancer patients.