In Vitro Biological Characterization of DCUN1D5 in DNA Damage Response

Abstract

<b>Background:</b> Novel prognostic biomarkers or therapeutic molecular targets for laryngeal squamous cellcarcinoma (LSCC) are an urgent priority. We here sought to identify multiple novel LSCC-associated genes.<br/><b>Methods</b>: Using high-density microarray expression profiling, we identified multiple genes that were significantlyaltered between human LSCCs and paired normal tissues. Potential oncogenic functions of one such gene,DCUN1D5, were further characterized in vitro. <br/><b>Results</b>: Our results demonstrated that DCUN1D5 was highlyexpressed in LSCCs. Overexpression of DCUN1D5 in vitro resulted in 2.7-fold increased cellular migration,67.5% increased invasive capacity, and 2.6-fold increased proliferation. Endogenous DCUN1D5 expression wasdecreased in a time-dependent manner after genotoxic stress, and silencing of DCUN1D5 by siRNA decreasedthe number of cells in the S phase by 10.2% and increased apoptosis by 11.7%. <br/><b>Conclusion</b>: Our data suggestthat DCUN1D5 in vitro might have vital roles in DNA damage response, but further studies are warranted toassess its significance in vivo.