Integration Sites and Genotype Distributions of Human Papillomavirus in Cervical Intraepithelial Neoplasia

Abstract

<br/><b>Objectives</b>: To analyse HPV integration prevalence and genotype distributions in cervical intraepithelialneoplasia (CIN) in east part of China, furthermore to assess preferential sites for common HPV integrations andprovide baseline information for cervical abnormality screening and prevention. <br/><b>Methods</b>: Integration of HPV in113 paraffin-embedded cervical intraepithelial neoplasia samples was assessed using Gencap technology in KeyLaboratory of Biotechnologies in BGI-Shenzhen. <br/><b>Results</b>: 64 samples were HPV-integrated and as the cervicallesions increased, the integration rate became higher significantly (P=0.002). Fifteen different HPV genotypeswere detected, 14 high-risk (16, 18, 31, 33, 51, 52, 56, 58, 66, 68) and 1 low-risk (11). The most common genotypeswere HPV-16, 58, 33, 52, 66, and 56. Thirteen patients had co-integration involving mainly HPV-16 and 58. Thefrequency of HPV gene disruption was higher in L1 and E1 genes than in other regions of the viral genomes.<br/><b>Conclusion</b>: Some 56.6% of CIN lesions in Qingdao had HPV integrations, and 67.2% of HPV-integrated patientswere HPV-16 and 58, more prone to be integrated in younger patients below 45 years old. There exist preferentialsites for HPV-16 and HPV-58 integration, and they are more likely to be disrupted in the L1 and E1 loci.