نمایش مختصر رکورد

dc.contributor.authorAmri, Jamalen_US
dc.contributor.authorMolaee, Nedaen_US
dc.contributor.authorKarami, Hadien_US
dc.date.accessioned1399-07-08T18:06:10Zfa_IR
dc.date.accessioned2020-09-29T18:06:10Z
dc.date.available1399-07-08T18:06:10Zfa_IR
dc.date.available2020-09-29T18:06:10Z
dc.date.issued2019-11-01en_US
dc.date.issued1398-08-10fa_IR
dc.date.submitted2019-01-27en_US
dc.date.submitted1397-11-07fa_IR
dc.identifier.citationAmri, Jamal, Molaee, Neda, Karami, Hadi. (2019). Up-Regulation of MiRNA-125a-5p Inhibits Cell Proliferation and Increases EGFR-TKI Induced Apoptosis in Lung Cancer Cells. Asian Pacific Journal of Cancer Prevention, 20(11), 3361-3367. doi: 10.31557/APJCP.2019.20.11.3361en_US
dc.identifier.issn1513-7368
dc.identifier.issn2476-762X
dc.identifier.urihttps://dx.doi.org/10.31557/APJCP.2019.20.11.3361
dc.identifier.urihttp://journal.waocp.org/article_88814.html
dc.identifier.urihttps://iranjournals.nlai.ir/handle/123456789/36267
dc.description.abstractBackground: Despite the dramatic efficacy of erlotinib, an EGFR tyrosine kinase inhibitor (TKI), most of non-small cell lung cancer (NSCLC) patients ultimately acquire resistance to this agent. Different studies indicated that miRNA-125a-5p is down-regulated in human lung cancer cells and may function as a tumor suppressor by targeting EGFR. However, the biological function of miRNA-125a-5p in NSCLC resistance to EGFR-TKIs is not fully understood. In this study the effect of miRNA-125a-5p on cell proliferation, apoptosis and sensitivity of the A549 lung cancer cells to erlotinib was investigated. Methods: After miRNA-125a-5p transfection, the expression levels of EGFR mRNA were measured by QRT-PCR. Trypan blue assays were performed to evaluate the proliferation of the A549 lung cancer cells. The cytotoxic effects of miRNA-125a-5p and erlotinib, alone and in combination, were determined using MTT assay. Combination index study was performed using the method of Chou-Talalay. Apoptosis was assessed using an ELISA cell death assay kit. Results: MiRNA-125a-5p clearly reduced the expression of EGFR mRNA in a time dependent manner, causing marked cell proliferation inhibition and spontaneous apoptosis (p<0.05, relative to control). Pretreatment with miRNA-125a-5p synergistically increased the cytotoxic effect of erlotinib and decreased its IC50. Furthermore, miRNA-125a-5p significantly enhanced the apoptotic effect of erlotinib. Negative control miRNA had no significant effect on biological parameter of the tumor cells. Conclusions: Our data suggest that suppression of EGFR by miRNA-125a-5p can effectively trigger apoptosis and overcome EGFR-TKs resistance of lung cancer cells. Therefore, miRNA-125a-5p may be a potential therapeutic adjuvant in patients with lung cancer.<br />en_US
dc.format.extent414
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoen_US
dc.publisherWest Asia Organization for Cancer Prevention (WAOCP)en_US
dc.relation.ispartofAsian Pacific Journal of Cancer Preventionen_US
dc.relation.isversionofhttps://dx.doi.org/10.31557/APJCP.2019.20.11.3361
dc.subjectApoptosisen_US
dc.subjectEGFRen_US
dc.subjecterlotiniben_US
dc.subjectLung canceren_US
dc.subjectMiRNA-125a-5pen_US
dc.subjectMolecular and cellularen_US
dc.titleUp-Regulation of MiRNA-125a-5p Inhibits Cell Proliferation and Increases EGFR-TKI Induced Apoptosis in Lung Cancer Cellsen_US
dc.typeTexten_US
dc.typeResearch Articlesen_US
dc.contributor.departmentMolecular and Medicine Research Center, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran.en_US
dc.contributor.departmentDepartment of Molecular Medicine and Biotechnology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran.en_US
dc.contributor.departmentMolecular and Medicine Research Center, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran.en_US
dc.citation.volume20
dc.citation.issue11
dc.citation.spage3361
dc.citation.epage3367
nlai.contributor.orcid0000-0002-8824-8160


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