Benzochloroporphyrin Derivative Induced Cytotoxicity and Inhibition of Tumor Recurrence During Photodynamic Therapy for Osteosarcoma

Abstract

Photodynamic therapy (PDT) is a promising cancer treatment modality that uses dye-sensitized photooxidationof biologic matter in target tissue. This study explored effects of the photosensitizer BCPD-17 during PDT forosteosarcoma. LM-8 osteosarcoma cells were treated with BCPD-17 and cell viability after laser irradiationwas assessed in vitro with the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. The effectsof BCPD-17 during PDT recurrence were then examined on tumor-bearing mice in vivo. BCPD-17 had dosedependentcytotoxic effects on LM-8 osteosarcoma cells after laser irradiation which also had energy-dependenteffects on the cells. The rate of local recurrence was reduced when marginal resection of mice tumors was followedby BCPD-17-mediated PDT. Our results indicated BCPD-17-mediated PDT in combination with marginalresection of tumors is a potentially new effective treatment for osteosarcoma.