Inhibitory Effect of Isolated Lactic Acid Bacteria from Scomberomorus commerson Intestines and their Bacteriocin on Listeria innocua

Abstract

ABSTRACT- Lactic acid bacteria are commonly found in the gastrointestinal tract of various endothermic animals and humans, in milk, dairy and seafood products and on some plant surfaces. The aim of this study was to investigate the inhibitory effect of isolated lactic acid bacteria from intestines of narrow-barred Spanish mackerel (Scomberomorus commerson) on Listeria innocua growth. Five strains of lactic acid bacteria (LAB) were isolated. Lactobacillus buchneri, Lactococcus lactis, Lactobacillus acidophilus, Lactobacillus fermentum and Sterptococcus salivarius strains were presumptively identified by biochemical and physiological tests. Anti-listerial activities of these isolates and their cell free supernatants (CFS) were evaluated using agar spot tests, agar well diffusion assays and BHI broth. The results revealed that all cultures and their cell free supernatants were able to inhibit L. innocua growth. Furthermore, the study showed that wastes of this kind of warm water sea fish, have antagonistic activities against L. innocua and can be potentially used as a free source of LAB.