Canola Oil Influence on Azoxymethane-induced Colon Carcinogenesis, Hypertriglyceridemia and Hyperglycemia in Kunming Mice

Abstract

Azoxymethane (AOM) is a potent genotoxic carcinogen which specifically induces colon cancer. Hyperlipidemiaand diabetes have several influences on colon cancer development, with genetic and environmental exposureaspects. Here, we investigated plasma lipid and glucose concentrations in Kunming mice randomized into fourgroups; control (no AOM or oil exposure), AOM control, AOM + pork oil, and AOM + canola oil. Aberrantcrypt foci (ACF), plasma cholesterol, plasma triglyceride, plasma glucose and organ weight were examined 32weeks after AOM injection. Results revealed that AOM exposure significantly increased ACF number, plasmatriglyceride and glucose level. Further, male mice displayed a much higher plasma triglyceride level than femalemice in the AOM control group. Dietary fat significantly inhibited AOM-induced hypertriglyceridemia, andcanola oil had stronger inhibitory effect than pork oil. AOM-induced hyperglycemia had no sex-difference andwas not significantly modified by dietary fat. However, AOM itself not change plasma cholesterol level. AOMsignificantly increased liver and spleen weight in male mice, but decreased kidney weight in female mice. Onthe other hand, mice testis weight decreased when fed canola oil. AOM could induce colorectal carcinogenesis,hypertriglyceridemia and hyperglycemia in Kunming mice at the same time, with subsequent studies requiredto investigate their genome association.