Association of Autophagy Gene ATG16L1 Polymorphism with Human Prostate Cancer and Bladder Cancer in Turkish Population

Abstract

Background: Urological cancers (prostate cancer and bladder cancers) are the most common cancers in Western<br />population and its rate is increasing in the Eastern World. Autophagy has appeared as a fundamental repair mechanism<br />for degrading damaged organelles and proteins. It was clear that autophagy gene polymorphisms are correlated with<br />development of inflammatory bowel disease and it can also be related with prostate cancer (PCa) or bladder cancer<br />(BCa). In this study, we aimed to determine if ATG16L1 (Thr300Ala) polymorphism is associated with an increased risk<br />of developing PCa and BCa and to establish correlations between ATG16L1 genotypes and morphological parameters.<br />Methods: This study included 269 healthy controls and 131 patients (62 PCa and 69 BCa) with PCa and BCa. The<br />ATG16L1 (rs2241880) gene regions were amplified using polymerase chain reaction (PCR), detected by restriction<br />fragment length polymorphism (RFLP). Results: At the end of our research, we found out that the genotype AG was<br />prevalent on patients and controls (34% vs 42%), followed by genotypes AA (35% vs 27%) and GG (31% vs 31%) in<br />PCa. The prevalence of genotypes of AA (wild-type), AG (heterozygous mutant) and GG (homozygous mutant) profiles<br />for the ATG16L1 Thr300Ala polymorphism were 35%, 40% and 25% respectively in BCa patients, and 32%, 40%<br />and 28% respectively in healthy control groups. The G allele frequency was 0.53 for in BCa patients and the control<br />groups. Conclusion: No association was found between ATG16L1 (Thr300Ala) polymorphism and patients with PCa<br />and BCa in Turkish population we studied.