Evaluation of Chemical Characteristics and Effects of Different Manganese Sources on Kinetics of Manganese Absorption and Performance of Broiler Chickens

Abstract

Three experiments wereconducted to evaluate chemical characteristics, intestinal absorption and bioavailability of manganese(Mn)from organic, inorganic and nano sources of Mn. In experiment 1, inorganic sources of Mn including Mn-sulphate and Mn-oxide, organic sources of Mn as Mn-glycinate and Mn-bioplex and FRA® easy dry Mn as a nano source of Mn were subjected to elemental analysis and solubility in deionized water, 0.4% hydrochloric acid, 2% citric acid and neutral ammonium citrate. In the experiment 2, intestinal absorption of Mn from these sources was investigated by <em>in vitro</em> everted gut sacs technique in broiler chicks. In the experiment 3, the bioavailability of Mn-sulphate, Mn-Glycinate and FRA® easy dry Mn was determined in chicks fed a corn-soybean meal-basal diet that was supplemented with 0, 40, 100, and 160 mg of Mn from these sources per kg of diet based on body weight gain (BWG), feed intake (FI) and feed conversion ratio (FCR) for 21 days from d 7 to 28. The results showed Mn-sulfate dissolved completely in all solvents. The solubility of all Mn sources was the lowest and the highest in deionized water and neutral ammonium citrate, respectively. The uptake percentages of Mn as nano Mn and Mn-oxide were significantly the highest and the lowest by duodenal and jejunal sacs, respectively. Mn as either Mn-Glycinate or nano Mn was absorbed more efficiently than Mn from other sources by ileal sacs. Amoung organic and inorganic sources, Mn-Glycinate and Mn-sulfate had the higher Mn absorption, respectively. BWG, FI and FCR did not affect by either Mn level or source. We concluded that ileum was the main site of Mn absorption for broilers and among different Mn sources, Mn-Glycinate and nano Mn had the highest Mn absorption. Furthermore, growth was not appropriate criteria to assess bioavailability of different Mn sources.