The Effect of Different Level of Concentrates in Diet on Microbial Protein Synthesis in Iranian Native Buffaloes

Abstract

This research was conducted to estimate the amount of microbial protein synthesis in Iranian buffaloes' rumen. Four male swamp buffaloes with average live body weight of 140±10 kg were used in this study. Four diets of 0 (control diet, 100% forage), 15, 30 and 45% concentrate in a 4 × 4 Latin Square were tested. The results indicated that by increasing the concentrate level in the diet of swamp buffaloes. the amount of purine derivatives (PD) excreted in urine. The amount of allantoin excreted in urine increased by increasing concentrate level form 0 to 45% (13.2 and 21.8 mmol/day; P<0.01). There was no significant difference between uric acid excretion from buffaloes fed with diets contained 15 and 30% or 30 and 45% concentrate (P>0.01). However, there was a significant difference between diets containing 45 and 15% concentrate level (P<0.01). The difference between control diet and other diets containing different levels of concentrates were significant (P<0.01). The total purine derivatives excretion and microbial nitrogen synthesis in rumen increased from 14.5 in control diet to 18.11, 21.58 and 24.55 mmol/day and from 38.76 in control diet to 60.44, 81.48 and 99.43 g/day in buffalos fed diets containing 15, 30 and 45% concentrate, respectively. The results of this experiment indicated that increasing the concentrate level in diet of swamp buffaloes increases the amount of microbial protein synthesized in rumen.