Interleukin-10 (1082G/A) Polymorphism is Associated with Susceptibility of Acute Myeloid Leukemia Patients in Sudanese Population

Abstract

Background: Interleukin-10 (IL-10) is a multifunctional cytokine with both immunosuppressive and antiangiogenicfunctions<br />and may have both tumor-promoting and -inhibiting properties. We examined the association<br />between a single nucleotide polymorphism (SNP) in IL-10 -1082G/A (rs1800896) in Sudanese acute myeloid leukemia<br />(AML) patients and to assess the association between polymorphisms in IL-10 -1082G/A (rs1800896) and the<br />hematological profile in Sudanese patients with AML. Methods: A total of 30 patients with acute myeloid leukemia<br />and 30 control subjects were enrolled in this study. Blood samples were collected from all patients in EDTA containing<br />tubes. Genomic DNA was extracted from all blood samples using salting out method. The genotypic variants of<br />IL-10 (-1082G/A) polymorphism were detected by allele specific-PCR. Results: We found that (36.7%) of patients have<br />homogenous GG genotype, (43.3%) have heterogeneous GA genotype and (20.0%) have AA genotype. GA genotype<br />was significantly associated with higher risk of AML compared with the homozygous Genotypes (GG and AA), there is<br />no association between IL-10 (-1082G/A) polymorphism and AML sub-type, gender, age group, mean of hematological<br />parameters. Conclusion: Our study concluded that GA genotype of IL-10 -1082G/A (rs1800896) polymorphism is a<br />risk factor for AML and G allele is insignificantly higher than A allele in AML patient. No association between IL-10<br />(-1082G/A) polymorphism and AML sub-type, gender, age group, mean of hematological parameters.