The Effect of Platelet Rich Plasma on Chondrogenic Differentiation of Human Adipose Derived Stem Cells in Transwell Culture

Abstract

<em><span style="font-size: xx-small;">Objective(s): </span></em> <span style="font-family: Cambria,Cambria; font-size: xx-small;">Platelet-rich plasma (PRP) has recently emerged as a promising strategy in regenerative medicine due to its multiple endogenous growth factors. Little is known about the role of PRP as a promoter in chondrogenesis of human adipose derived stem cells (hADSCs). The aim of this study was to determine whether PRP may be considered as a natural and easy achievable source of growth factors to promote the chondrogenic differentiation of hADSCs in Transwell culture. </span>

Materials and Methods <em><span style="font-size: xx-small;"><span style="font-family: Times New Roman,Times New Roman; font-size: xx-small;"><em><span style="font-family: Times New Roman,Times New Roman; font-size: xx-small;">: </span></em></span></span></em><span style="font-family: Cambria,Cambria; font-size: xx-small;"><span style="font-family: Cambria,Cambria; font-size: xx-small;">Biochemical, immunohistological and molecular assays were used to evaluate the effect of different concentrations (5%, 10%, and 15%) of PRP on chondrogenic differentiation of hADSCs in Transwell culture. </span></span> Results <em><span style="font-size: xx-small;"><span style="font-family: Times New Roman,Times New Roman; font-size: xx-small;"><em><span style="font-family: Times New Roman,Times New Roman; font-size: xx-small;">: </span></em></span></span></em><span style="font-family: Cambria,Cambria; font-size: xx-small;"><span style="font-family: Cambria,Cambria; font-size: xx-small;">The cells in the presence of 10% PRP produced markedly higher amounts of GAG and DNA, in comparison to the control group. PRP also increased chondrogenic markers in these cells, such as sox-9, aggrecan and collagen type II. A high expression level of collagen type X as a hypertrophic marker was observed in cartilage produced by using either PRP or TGF-β1. </span></span> Conclusion <em><span style="font-size: xx-small;"><span style="font-family: Times New Roman,Times New Roman; font-size: xx-small;"><em><span style="font-family: Times New Roman,Times New Roman; font-size: xx-small;">: </span></em></span></span></em><span style="font-family: Cambria,Cambria; font-size: xx-small;"><span style="font-family: Cambria,Cambria; font-size: xx-small;">Our findings indicate that autologous PRP at an optimum concentration had beneficial effects on differentiation of hADSCs in Transwell culture. Further, </span></span><em><span style="font-family: Cambria,Cambria; font-size: xx-small;"><em><span style="font-family: Cambria,Cambria; font-size: xx-small;">in vivo </span></em></span></em><span style="font-family: Cambria,Cambria; font-size: xx-small;"><span style="font-family: Cambria,Cambria; font-size: xx-small;">studies are necessary to fully define the clinical implications of PRP. </span></span>