c-Src Antisense Complexed with PAMAM Denderimes Decreases of c-Src Expression and EGFR-Dependent Downstream Genes in the Human HT-29 Colon Cancer Cell Line

Abstract

c-Src is one member of non-receptor tyrosine kinase protein family that has over expression and activationin many human cancer cells. It has been shown that c-Src is implicated in various downstream signalingpathways associated with EGFR-dependent signaling such as MAPK and STAT5 pathways. Transactivation ofEGFR by c-Src is more effective than EGFR ligands. To inhibit the c-Src expression, we used c-Src antisenseoligonucleotide complexed with PAMAM Denderimes. The effect of c-Src antisense oligonucleotide on HT29cell proliferation was determined by MTT assay. Then, the expression of c-Src, EGFR and the genes related toEGFR-depended signaling with P53 was applied by real time PCR. We used western blot analysis to elucidate theeffect of antisense on the level of c-Src protein expression. The results showed, c-Src antisense complexed withPAMAM denderimers has an effective role in decrease of c-Src expression and EGFR-dependent downstreamgenes.