Repetitive sequences based on genotyping of Candida albicans isolates obtained from Iranian patients with human immunodeficiency virus

Abstract

<em>Objective(s):</em> Candidiasis infection caused by <em>Candida albicans</em> has been known as a major problem in patients with immune disorders. The objective of this study was to genotype the <em>C. albicans</em> isolates obtained from oral cavity of patients with positive human immunodeficiency virus (HIV⁺) with or/and without oropharyngeal candidiasis (OPC). <br/><em>Materials and Methods:</em>A total of 100 <em>C. albicans</em> isolates from Iranian HIV⁺patients were genotyped using specific PCR primers of the 25S rDNA and RPS genes. <br/><em>Results:</em> The frequencies of genotypes A, B and C which were achieved using 25S rDNA , were 66, 24 and 10 percent, respectively. In addition, genotypes D and E were not found in this study. Each <em>C. albicans</em> genotype was further classified into four subtypes (types 2, 3, 2/3 and 3/4) by PCR amplification targeting RPS sequence. <br/><em>Conclusion:</em>In general, genotype A3 constituted the majority of understudy clinical isolates obtained from oral cavity of Iranian HIV⁺ patients.