Transcriptional regulation of E-cadherin and oncoprotein E7 by valproic acid in HPV positive cell lines

Abstract

<strong><em>Objective(s):</em></strong> Valproic acid (VPA) has proven to be as one of the most promising useful drug with anticancer properties.In this study, we investigate the VPA effects on E-cadherin expression in HeLa, TC₁, MKN_45, and HCT₁₁₆ cell lines. This study assesses the effects of VPA on human papillomavirus E7 expression in HPV positive cell lines. <strong><em>Materials and Methods:</em></strong> Cell lines were treated by2 mmol/l VPA and expression of E-cadherin and E7 was analyzed by quantitative real-time PCR. Student's <em>t</em> test and ANOVA were used to determine changes in expression levels. <strong><em>Results:</em></strong>The results revealed that mean of E-cadherin expression is increased by VPA 1.8 times in HCT₁₁₆ and MKN₄₅ cell lines, also the mean of E-cadherin mRNA levels is up-regulated 2.9 times in HeLa and TC₁ cell lines. So, E-cadherin augmentation induced by VPA in HeLa and TC-1, HPV positive cell lines, is higher than HPV negative cell lines MKN₄₅ and HCT₁₁₆. The mean of HPV E7 expression is decreased by VPA, 4.6 times in in HeLa and TC-1 cell lines. <strong><em>Conclusion: </em></strong>This study demonstrates that re-expression of E-cadherin by VPA in HPV positive cell lines is more than HPV negative cell lines. Whereas, HPV E7 reduces the expression of E-cadherin, reduction of HPV E7 expression by VPA is related to more augmentation of E-cadherin in HPV positive cell lines. So, this study demonstrates that VPA has more anticancer properties in HPV positive cell lines, and could potentially be a promising candidate for cervical cancer treatment.