Opium induces apoptosis in Jurkat cells via promotion of pro-apoptotic and inhibition of anti-apoptotic molecules

Abstract

<strong><em>Objective(s):</em></strong> The aim of this study was to determine the important molecules involved in apoptosis induction by opium in Jurkat cell line. <strong><em>Materials and Methods:</em></strong> Jurkat cells were incubated 48 hrs with2.86×10^-5 g/ml concentration of opium and apoptosis as well as expression levels of related molecules weremeasured. <strong><em>Results: </em></strong>Our results demonstrated that 50.3±0.2 percent of opium treated Jurkat cells were revealed apoptotic features. The levels of mRNA of several pro-apoptotic and anti-apoptotic molecules were increased and decreased, respectively, in the opium treated cells. The results also demonstrated that expression levels of BCL2, DFFA and NOL3 as anti-apoptotic molecules were increased in the opium treated cells. <strong><em>Conclusion:</em> </strong>It seems that opium induces apoptosis in Jurkat cells via both intrinsic and extrinsic pathways. Although opium induces apoptosis in the cells but increased expression of some anti-apoptotic molecules may be a normal resistance of the cell for death.<strong> </strong>