Evaluation of Tnf-α and Il-6 mRNAs expressions in visceral and subcutaneous adipose tissues of polycystic ovarian rats and effects of resveratrol

Abstract

<strong><em>Objective(s)</em></strong>: Some studies suggest that chronic low-grade inflammation is involved in insulin resistance in polycystic ovary syndrome (PCOS). This study assessed possible involvement of alteration in expression of two pro-inflammatory factors, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in adipose tissues of PCOS rats in the impairment of insulin actions. Also, effects of resveratrol as an anti-inflammatory agent were investigated.<br /> <strong><em>Materials and Methods: </em></strong>Fifteen female <em>Wistar</em> rats (21 days old) were divided into three groups (n=5): Ι) Control, П) PCO-model-saline: served as PCOS rats and to induce PCOS, received subcutaneously testosterone enanthate 1 mg/100 g body weight subcutaneously for 35 days, Ш) PCO-model-resveratrol, after receiving testosterone, received resveratrol 10 mg/kg intraperitoneally for 28 days. The expression of <em>Tnf-α </em>and<em> Il-6 </em>mRNAs in adipose tissues was determined by the qRT-PCR method.<br /> <strong><em>Results:</em></strong> The <em>Il-6</em> mRNA expression in the visceral adipose tissue of PCOS rats was increased in comparison to controls (<em>P</em><0.05). <em>Tnf-α</em> and <em>Il-6 </em>mRNA expression in visceral and subcutaneous adipose tissues of polycystic ovarian rats was similar to controls. The expression of <em>Tnf-α</em> mRNA in subcutaneous adipose tissue and <em>Tnf-α</em> and <em>Il-6 </em>mRNAs in the visceral adipose tissue of the PCO-model-resveratrol group were lower than PCOS rats (<em>P</em><0.05).<br /> <strong><em>Conclusion:</em></strong> Increased expression of <em>Il-6</em> mRNA in the visceral adipose tissue of polycystic ovarian rats may be one cause of insulin resistance observed in them and resveratrol as an anti-inflammatory and anti-hyperglycemic agent may decrease the risk of diabetes by reduction of expression of pro-inflammatory cytokines TNF-α and IL-6 in PCOS patients.