Production of Single Cell Protein from Sugarcane Bagasse by Saccharomyces cerevisiae in Tray Bioreactor

Abstract

In this study, solid state fermentation (SSF) was carried out to produce single cell protein (SCP) from sugarcane bagasse using Saccharomyces cerevisiae. The SSF experiment were performed in a tray bioreactor. The influence of several parameters including extraction buffer, initial moisture content of substrate, fermentation time, relative humidity in bioreactor, the bioreactor temperature and pretreatment of substrate on SCP production yield was considered. Among the used extraction buffers, carbonate-bicarbonate buffer was the most effective one for protein extraction. The obtained results revealed that the suitable fermentation conditions were initial substrate moisture content of 70%, fermentation time of 72 h, relative humidity of 85%, bioreactor temperature of 35 oC and pretreatment of substrate using 2% NaOH solution; at this optimum condition protein production yield of 13.41% was attained. The amino acid analysis of the produced protein showed that the product contained almost all of the essential amino acid as well as some non-essential amino acids.