Impact of Salicylic Acid on Phenolic Metabolism and Antioxidant Activity in Four Grape Cultivars during Cold Storage

Abstract

Salicylic acid (SA) plays an important role in the regulation of plant ripening and responses to abiotic stresses. In this study, the protective effect of SA on cold stress-caused oxidative damage in grape (<em>Vitis vinifera </em>L<em>.</em>) bunches was investigated during cold storage. Grape bunches treated with 2 mM SA and stored at 0°C with 85-90% RH for 30 days. Samples were selected from each treatment for physical and chemical analysis before storage, midpoint of storage, end of storage and end of shelf life. The results showed that the cold storage induced lipid peroxidation, decay incidence and loss of fresh weight, while SA pretreatment mitigated these effects in “Bidaneh Sefid" and “Qzl uzum" cultivars. In these cultivars, SA-induced protection against cold damage was accompanied by decrease in malondialdehyde (MDA) content, and caused an increase in catalase (CAT) and superoxide dismutase (SOD) activity. Additionally, SA-induced protection against cold injury was mediated through promoting total phenolics concentration and phenylalanine ammonia-lyase (PAL) activity. The present findings suggest that although variability in the protective effect of SA against cold damage was observed between grape cultivars, SA could alleviate the cold-induced oxidative stress in grape bunches during cold storage.