In Vitro Regeneration of Iranian Melon (Cucumis Melo L. ‘Samsoori’) Using Antibiotic and Benzyl adenine Micropropagation of Cucumis Melo L. ‘Samsoori’

Abstract

<em>Cucumis melo</em> L. is one of the most economically important horticultural crops worldwide. However, low plant regeneration frequency of this plant during genetic transformation is the major hurdle for applying biotechnological approaches. Hence, this study aimed to evaluate the effect of 6-benzyladenine (BA), cefotaxime (CTX), kanamycin (KAN), and indole-3-acetic acid (IAA) on the regeneration of cotyledonary petioles generated from 6-day-old <em>in vitro</em> grown seedlings. Results showed that application of 1.5 mgl^-1BA plus 250 mgl^-1CTX and 1 mgl^-1 BA with 1000 mgl^-1 CTX formed the most efficient media for plant regeneration. The highest callus production was recorded on medium containing 1 mgl^-1 BA with 250 mgl^-1 CTX and 1.5 mgl^-1 BA with 750 mgl^-1 CTX. Medium containing 500 mgl^-1CTX plus 0.1 mgl^-1IAA efficiently induced both root and leaf formation. All regenerated plants were died by adding 100 mgl^-1 kanamycin therefore this level considered as threshold level for kanamycin application.. Overall, the results indicated that presence of BA plays an essential role for melon regeneration and cefotaxime can be considered as an auxiliary agent.