IL-6, IL-10 and IL-17 Gene Polymorphisms in Iranian Women with Recurrent Miscarriage

Abstract

<b>Background</b>: <span>Pro</span><span>-</span><span>inflammatory and anti-inflammatory cytokines and polymorphisms</span> of their genes have been described to be involved in the pathogenesis of recurrent miscarriage (RM). <span><br/><b>Objective</b>: </span><span>To investigate the association between RM and five</span> polymorphisms of cytokine genes, interleukin 10 <span>(</span><span>IL-10), (-592 A/C, -819 C/T, -1082</span> A/G), IL-6 (-174 C/G) and IL-17 (-197 G/A) in Iranian women. <span><br/><b>Method</b>: </span><span>Polymerase</span> chain reaction <span>-</span><span>restriction fragment length polymorphism (PCR-RFLP) was performed</span> to determine the frequencies of the IL-6, IL-10 and IL-17 gene polymorphisms in 85 women with RM compared with 104 healthy controls. <span><br/><b>Results</b>: </span><span>The frequencies of IL-</span> 10 promoter gene polymorphisms (-592 A/C and -819 C/T) were significantly higher in RM women than those in controls (p=0.003). However, no statistically significant differences were observed in the frequencies of IL-6 (-174 C/G), IL-10 (-1082 A/G) and IL-17 (-197 G/A) polymorphisms between RM women and controls. <br/><b>Conclusion</b>:<span> These</span> results suggest that IL-10 gene polymorphism screening might have some relevance in patients with RM, a suggestion which requires further studies.