Evaluation of antibiotic resistance pattern in Staphylococcus saprophyticus isolated from patients with urinary tract infection using real-time PCR

Abstract

<em>Staphylococcus saprophyticus</em> is an important agent of urinary tract infections (UTIs), especially among young women. The aim of this study was to identify <em>S. saprophyticus</em> as a common cause of urinary tract infections and determine their antibiotic susceptibility. In this study, 51235 clinical samples were collected from therapeutic centers of Mazandaran. <em>S. saprophyticus</em> was confirmed by the real- time PCR technique through <em>rrs</em> gene and their antibiotic resistance pattern determined by disc diffusion method. Of the 51235 cultivated samples, only 2101 (4.1%) cases had significant bacteriuria. In this study, the prevalence of <em>S. saprophyticus</em> was 2.47%. <em>Escherichia coli</em> was the most common bacterium isolated from patients with UTIs at the rate of 61.06%. <em>S. saprophyticus</em> showed the highest frequency of antibiotic resistance to erythromycin at the rate of 80% and ampicillin and cefotaxime were ranked with the frequency of 55 and 17.5%, respectively. The present study shows that bacterial resistance is a potential problem in Mazandaran province.