PCR–RFLP of isolated Salmonella from poultry with Sau3AI and HhaI restriction endonucleases in Arak

Abstract

<em>Salmonella</em> <em>enterica</em> is considered as one of the major pathogens in public health, worldwide. Diseases caused by <em>Salmonella enterica</em> serovars are especially prevalent in developing countries. It is one of the emerging pathogen in food-borne diseases, which is often found in contaminated chicken eggs. The aim of this study was to apply a PCR–RFLP method using Sau3AI and HhaI restriction endonucleases on the fliC gene to identify the serotypes of <em>Salmonella</em> isolates in avian living in Arak area in central Iran. Serotypes of 75 isolates included <em>Salmonella</em> <em>enteritidis</em> (45.33%), <em>Salmonella infantis</em> (44%), <em>Salmonella</em> <em>typhimurium</em> (5.33%), <em>Salmonella bardo</em> (2.67%) and <em>Salmonella bacongo</em> (2.67%). All the isolates showed fliC gene (1500bp) by using specific primers. The results of PCR-RFLP with restriction enzymes HhaI and Sau3AI for gene fliC showed 4 and 5 patterns. It was observed that Sau3AI is able to discriminate all serotypes of <em>Salmonella</em> but HhaI could not distinguish <em>Salmonella infantis</em> and <em>Salmonella</em> <em>typhimurium</em>. The prevalent <em>Salmonella</em> isolated in this study belonged to serotype enteritidis and infantis. The combination of the data obtained with both restriction enzymes could differentiate the serovars completely. These results showed that fliC gene is a suitable target gene for discriminating among these <em>Salmonella</em> serotypes by PCR-RFLP.