Identification and Screening of Homozygous and Heterozygous Almond Progenies from Self-Pollinated Touno Cultivar Using PCR

Abstract

Self-incompatibility is one of the most important difficulties in almond production which reduce fruit set dramatically and makes orchard management difficult. Therefore, breeding almond to produce self-compatible genotypes is very important. In this research identification and screening of 86 almond progenies obtained from selfing Touno after the self-pollination by PCR reaction with specific primers of CEBAS_f and AS1. PCR results confirmed the situation of self-compatible hybrids. In addition, it indicated that, frequencies of S_f, and S₁ was 100% and 50% in progenies respectively. Self-compatible hybrids had been identified that can be used in almond breeding programs particularly to development the monoculture of almond orchards. So to identify and screening homozygous self-compatibility almonds be capable of be another step towards creating monoculture of almond and use in breeding programs further.