The Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs) Genes among Clinical Isolates of Staphylococcus aureus from Hospitalized Children

Abstract

<em>Background:</em>Isolates of <em>Staphylococcus aureus</em> express a myriad of adhesive surface proteins that play important role in colonization of the bacteria on nasal and skin surfaces, beginning the process of pathogenesis. The aim of this study was to screen several of the Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs) genes among the isolate of <em>S. aureus</em> from hospitalized children. <em>Methods:</em>A totalof 22 <em>S. aureus</em> isolates were collected from hospitalized children in Tehran from 2012 to 2013. Detection of the <em>mecA</em> and several adhesive surface proteins genes including <em>clfA, B</em> (encoding clumping factors A, B); <em>fnbA</em>, <em>B</em> (encoding finronectin binding proteins A, B); <em>fib</em> (encoding fibrinogen binding protein); <em>eno</em> (encoding laminin binding protein); <em>cna</em> (encoding collagen binding protein); <em>ebps</em> (encoding elastin binding protein) and <em>bbp</em> (encoding bone sialo-protein binding protein), was performed by PCR. <em>Results:</em> The <em>clfAB</em> genes were detected among all the isolates. The prevalence of <em>fnbA</em>, <em>fnbB</em>, <em>fib</em>, <em>eno</em>, <em>cna</em>, <em>ebps</em> and <em>bbp</em> was 63%, 6%, 50%, 59%, 82%, 63%, 9% and 0%, respectively. <em>Conclusion</em><em>:</em> The high prevalence of these genes is important for future plans in vaccine designation. MRSA and MSSA isolates similarly can produce adhesive surface proteins for colonization.