Monocyte Chemoattractant Protein-1 Modulates Invasion and Apoptosis of PC-3M Prostate Cancer Cells Via Regulating Expression of VEGF, MMP9 and Caspase-3

Abstract

Prostate cancer is a form of malignancy that is most likely to develop in older males, but because of thepropensity to metastasize to parts of the body, particularly the bones, can have a deleterious impact on qualityof life. Recently monocyte chemoattractant protein-1 (MCP-1) has been shown to play important role in prostatecancer progression and metastasi. In this study we aimed to investigate the mechanisms underlying its functionalroles. In vitro transwell invasion assays with PC-3M prostate cancer cells demonstrated MCP-1 promotion ofinvasion, while annexin V-FITC and TUNEL confirmed inhibition of apoptosis. Treatment MCP-1 further ledto significant upregulation of VEGF and MMP-9 and downregulation of Caspase-3 at both mRNA and proteinlevels compared with untreated control (P<0.05), while siRNA mediated knockdown reversed these changes.Taken together, our results indicate important roles of MCP-1 in prostate cancer progression and metastasisand our finding of regulation of VEGF, MMP-9 and Caspase-3 expression open up new possibilities for targetedtherapy.