Gene Expression and Pulmonary Toxicity of Chitosan-graft-Polyethylenimine as Aerosol Gene Carrier
دریافت مدرک
FullText
اندازه فایل:
977.8کیلوبایتنوع فايل (MIME):
PDFنوع مدرک
TextResearch article
زبان مدرک
Englishچکیده
Chitosan-graft-polyethylenimine (CHI-g-PEI) copolymer has been used for theimprovement of low transfection efficiency of chitosan. The present study aims to test thepulmonary toxicity and efficiency of CHI-g-PEI as an aerosol gene carrier. Mice were exposedto aerosol containing green-fluorescent protein (GFP)-polyethylenimine (PEI) or GFP-CHIg-PEI complexes for 30 min during the development of our nose-only exposure chamber(NOEC) system. CHI-g-PEI-mediated aerosol delivery demonstrated 15.65% enhancementof the fluorescence intensity. Compared to PEI, CHI-g-PEI showed no significant pulmonarytoxicity. In summary, using CHI-g-PEI is safe and shows high transfection in aerosol genedelivery to animals, and enhanced efficiency was achieved through our aerosol gene deliverysystem. Therefore, CHI-g-PEI and this system would be applicable to future study for aerosolgene therapy.
کلید واژگان
Aerosol deliveryChitosan
Gene carrier
Polyethylenimine
Pulmonary toxicity
Medicinal chemistry
Pharmacotherapy (Clinical Pharmacy)
Pharmacutical biotechnology
toxicology and Pharmacology
شماره نشریه
2تاریخ نشر
2013-06-011392-03-11
ناشر
School of Pharmacy, Shahid Beheshti University of Medical Sciencesسازمان پدید آورنده
Laboratory of Toxicology, College of Veterinary Medicine, Seoul 151-742, Korea.1- Laboratory of Toxicology, College of Veterinary Medicine, Seoul 151-742, Korea. 2- School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China.
Laboratory of Toxicology, College of Veterinary Medicine, Seoul 151-742, Korea.
Nanotechnology and Thermal Processing Laboratory, School of Mechanical and Aerospace Engineering, Seoul 151-742, Korea.
Nanotechnology and Thermal Processing Laboratory, School of Mechanical and Aerospace Engineering, Seoul 151-742, Korea.
Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Korea.
Indang Institute of Molecular Biology, Inje University, Seoul 100-032, Korea.
1- Laboratory of Toxicology, College of Veterinary Medicine, Seoul 151-742, Korea. 2- Department of Nanofusion Technology, Graduate School of Convergence Science and Technology, Seoul National University, Suwon 443-270, Korea. 3- Advanced Institute of Convergence Technology, Seoul National University, Suwon 443-270, Korea.
شاپا
1735-03281726-6890
