Introducing Potential Key Proteins and Pathways in Human Laryngeal Cancer: A System Biology Approach

Abstract

The most common malignant neoplasm of the head and neck region is laryngeal cancerwhich presents a significant international health problem. The present study aims to screenpotential proteins related to laryngeal cancer by network analysis to further understandingdisease pathogenesis and biomarker discovery. Differentially expressed proteins were extractedfrom literatures of laryngeal cancer that compare proteome profiling of patient›s tissue withhealthy controls. The PPI network analyzed for up and down regulated proteins with CytoscapeVersion 3.4. After PPI construction, topological properties of the two networks have beenanalyzed. Besides, by using MCODE. the Gene Ontology (GO) analysis, the related modulesand pathways were examined. Our study screened 275 differentially changed proteins, including136 up- and 139 down-regulated proteins. For each network, it has been considered 20 keyproteins as hub and 20 as bottleneck. A number of 26 hub-bottleneck nodes is introduced forthe two networks. A total of 11 modules including 6 downregulated and 5 upregulated networkmodules were obtained. The most significant GO function in the significant upregulated modulewas the RNA processing, and the most significant one in the downregulated module withhighest score was the respiratory electron transport chain. Among 275 investigated proteins, 12crucial proteins are determined that 4 of them can be introduce as a possible biomarker panelincluding YWHAZ, PPP2R1A, HSP90AA1, and CALM3 for human laryngeal cancer.