Investigation of β-Sitosterol and Prangol Extracted from Achillea Tenoifolia Along with Whole Root Extract on Isolated Rat Pancreatic Islets
(ندگان)پدیدآور
Rahimifard, MahbanManayi, AzadehBaeeri, MaryamGholami, MahdiSeidnia, SoodabehAbdollahi, Mohammadنوع مدرک
TextResearch article
زبان مدرک
Englishچکیده
The genus Achillea (Asteraceae) consists of important medicinal species, growing wildly in Iran, of which A. tenuifolia is found in Iran-o-Turan regions. Regarding the traditional use of Achillea species for treatment of diabetes and also lack of information on phyto-constituents of A. tenuifolia underground parts, in this study the results of anti-diabetic activity of this plant have been reported. In order to find the main active components, underground parts of this plant were extracted with water and fractioned by hexane, ethyl acetate, and methanol and the separation of the main compounds were carried out via medium pressure liquid chromatography (MPLC). Also, anti-diabetic effects of the extract were investigated on rat pancreatic islets. The root extract of this plant as well as the compound β-sitosterol showed moderate α-amylase inhibitory activity but prangol did not suppress the enzyme activity. The results of islet cells' bio-function assays revealed that the herb root extract was able to increase the secretion of insulin in high concentration (10 mg/mL) and improved the cell viability with no toxicity in all doses. Furthermore, the herbal extract could reduce the levels of reactive oxygen species (ROS) and lipid peroxidation (LPO). The plant extract also significantly decreased the enzyme activity for both caspase-3 and -9 and increased the antioxidant capacity of the isolated cells. Taking together, preparations or extracts from the underground parts of this plant deserve a good candidate for further anti-diabetic investigation and clinical trials.
کلید واژگان
Achillea tenuifolia rootPrangol
β-Sitosterol
Pancreatic islet cells
Oxidative Stress
toxicology and Pharmacology
شماره نشریه
1تاریخ نشر
2018-01-011396-10-11
ناشر
School of Pharmacy, Shahid Beheshti University of Medical Sciencesسازمان پدید آورنده
Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 1417614411, IranMedicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 1417614411, Iran
Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 1417614411, Iran
Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 1417614411, Iran
Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 1417614411, Iran
Department of Toxicology and Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Science, Tehran, Iran
شاپا
1735-03281726-6890