The effect of olive extract on human neutrophils

Abstract

The neutrophil respiratory burst, which is essential for the host's ability to kill microorganisms, can be stimulated by some natural agents. Olea europaea (Oleaceae) is used as anti hypertensive, hyperglycemia, and hypercholestremia in traditional medicine. Here in this study, we evaluate the stimulatory effect of olive oil extract on oxidative burst in human neutrophils in vitro. Buffy coat was separated from heparinized blood from normal volunteers by sedimentation in 1% W/V dextran then overlaid on Ficoll-Hipaque and centrifuged for 30 min. After washing, the neutrophils were cultured in 96 well plates containing 0.2 ml RPMI1640 as medium. After treatment with different doses of olive oil extract, the wells were washed with HBSS then incubated with 0.1 ml NBT (1 mg/ml) and 0.1 ml RPMI1640. The reduced NBT was solubilised by adding DMSO then the OD of 630 nm was read using micro ELISA reader. Statistical analysis by ANOVA, tukey test showed that there is dose response effect in the range of doses that examined (p