Tissue distribution of artemisinin in broiler chickens following single or multiple oral administration

Abstract

<strong>Background:</strong> Artemisinin is commonly used for the treatment of malaria, but recently has been considered as a potential substance to control poultry coccidiosis. <strong>OBJECTIVES: </strong>The aim of the present study was to determine the tissue distribution of artemisinin following single or multiple oral administration of different doses in broiler chickens.<strong> METHODS: </strong>A total number of 390 one day old Ross broiler chicks were divided randomly into two main groups, in the first group 0, 1, 5, 25, 125, 250, 500 or 1000 mg/kg artemisinin as a single oral dose was administered on day 44, but the second group were treated with 0, 17, 34, 68 or 136 ppm artemisinin from day 8 to day 44. The HPLC system was used to determine the level of artemisinin in different tissue samples. Data were assessed using one way analysis of variance (ANOVA) followed by the Tukey's test (p<0.05). <strong>RESULTS: </strong>Maximum concentrations of artemisinin were found in the liver of chickens in both groups in a dose dependent manner. While, the minimum level was determined in the brain and the kidney of chickens received multiple artemisinin administration; in the spleen of those chickens a single oral dose was administered. The concentration of artemisinin in the brain reached a plateau at 68 ppm in multiple administration and 125mg/kg at single dose, no shift was found with dose increment. <strong>CONCLUSIONS:</strong> It can be concluded that tissue accumulation of artemisinin is time and dose dependent. Moreover, redistribution, saturation effect and tissue selectivity were also observed.