Measuring of free endotoxin in alum-precipitated vaccine of haemorrhagic septicaemia by limulus amebocyte lysate test

Abstract

Haemorrhagic septicaemia (HS) vaccine which is prepared in Razi Institute is used in endemic areas ofIran. Aluminum-hydroxide gel was used as adjuvant for preparing this vaccine. Post-vaccinal shock reactionswere the main complaint after use of this vaccine. In a previous study, we could improve the vaccine byalum-precipitation Pasteurella multocida cells and removing the liquid phase. In this study, the amount offree endotoxin in aluminum-hydroxide and alum-HS vaccines was determined. It was found that endotoxinlevel was considerably decreased from 0.22 EU/ml to 0.03 EU/ml after alum-precipitation.