Hyperimmune lipopolysaccharide antiserum mediated inhibition of the adherence of E. coli O157:H7 to HEP-2 cells and large intestine of mice

Abstract

Escherichia coli O157:H7 is found in cattle farms and can live in the intestine of healthy cattle. Mostcases of human illnesses including nonbloody diarrhea, hemorrhagic colitis and hemolytic uremic syndromecan be traced, either directly or indirectly, to cattle. One strategy for reducing the risk of EnterohemorrhagicEscherichia coli (EHEC) infections in human is to reduce the prevalence of infection in cattle. Antiserumagainst whole cell of isolated E. coli O157:H7 from cattle showed inhibition of adherence of this strain toHEP-2 cells and intestine of model mice. The ability of this antiserum in blocking the attachment of bacteriato HEP-2 cells in 1:1280 titer and to intestine tissue of mice in 1:640 titer are significant. Histology ofintestine tissue confirms our results. The difference between in vivo and in vitro titrations for blocking theattachment depends on these two different conditions.