Anticancer Effect of Cisplatin-Loaded Poly (Butylcyanoacrylate) Nanoparticles on A172 Brain Cancer Cells Line

Abstract

Background: Drug delivery systems have been designed to achieve targeted delivery and control the release rate<br />of the drugs. A serious challenge associated with drug delivery systems is the presence of the blood-brain barrier which<br />limits drugs penetration. In the current study, the effects of cisplatin nanoparticles on A172 brain cancer cell line were<br />investigated. Methods: Cisplatin nanoparticles were produced by miniemulsion polymerization technique and their<br />properties were evaluated. Drug release assay was performed to characterize the nanoparticles' properties. Here, we<br />examined the effects of cisplatin nanoparticles and free form of cisplatin on A172 cancer cell line. MTT assay was<br />performed for different concentrations of the drug. To measure the apoptosis rate in A172 cell line in the presence<br />of cisplatin nanoparticles or its free from, Annexin V staining method was used. Results: Our results indicated that<br />loading type of cisplatin was physical loading and only 4.7% of cisplatin was released after 68 h. Furthermore, MTT<br />assay showed that cisplatin nanoparticles in all concentrations had more cytotoxic effects on the cells comparing with<br />the free form of cisplatin and control groups. We also showed that cisplatin nanoparticles could increase apoptosis<br />in cancer cells more than the drug in the free form by using flow cytometry technique. Conclusion: Overall, these<br />findings proved that cisplatin loaded on poly (Butylcyanoacrylate) nanoparticles, was more efficient than the free form<br />of cisplatin in treating A172 cancer cell line.