Stromal Expression of CD10 by Immunohistochemistry in Odontogenic Keratocyst (OKC), Dentigerous and Radicular Cysts and Its Correlation with Local Recurrence and Aggressive Behaviour

Abstract

Objective: To assess and compare the stromal expression of CD10 in OKC, dentigerous and radicular cysts. Materials<br />and Methods: This comparative, cross sectional study was conducted at Armed Forces Institute of Pathology (AFIP),<br />Rawalpindi, from Jan 2017 to Dec 2017. Total sixty cases comprising 20 of each OKC, Dentigerous and Radicular cysts<br />were included in this study. Hematoxylin and eosin (H and E) sections were performed followed by immunohistochemical<br />staining for CD10 antibody. Expression of CD10 was evaluated and compared. Results were analyzed by using SPSS<br />version 20.0. Chi Square test was performed with P value < 0.05 was considered as significant. Results: A total of<br />60 cases, 20 of each OKC, dentigerous and radicular cysts were taken. In our study, 38 (63.3%) male and 22 (36.7%)<br />female patients with the mean age of 32 ± 15 (mean ± SD) were included. Percentage of CD10 positive cells were<br />highest in sub-epithelial stroma of OKC (95% cases) as compared to radicular and dentigerous cysts (60 and 70%) with<br />highest number of cases showing intense staining in OKC 13(65%) as compared to other odontogenic cysts i-e 4(20%)<br />and 2 (10%) respectively. There was a statistically significant association between odontogenic cysts and proportional<br />score, intensity score and combined score of stromal CD10 expression (P=0.009, p=0.001 and p=0.000). Conclusion:<br />In this study, we found that highest stromal CD10 expression in OKC as compared to dentigerous and radicular cyst,<br />which might be due to aggressive behaviour and increased risk of recurrence in OKC. Expression of CD10 marker will<br />further aid the clinician to plan appropriate surgical intervention and keep regular follow-ups to identify recurrences.