Production, formulation and quality control of [13N]NH3 compound for PET imaging of regional blood flow [Persian]

Abstract

In this study, 16O(p,α)13N nuclear reaction was chosen as the best method of N-13 production. High purity natural water targets were bombarded by 18 MeV protons with an intensity of 8μA. A production yield of 13.5 mCi/μAh was obtained. Chemical modification of 13N-nitrogen oxides into [13N]NH3 was accomplished using De Varda's catalyst. Chemical, radiochemical and radionuclide purity controls performed on the final product showed high radiopharmaceutical purity (>98%) in the form of [13N]NH3. [13N]NH3 was formulated in normal saline media at appropriate pH and sterile conditions. Microbial and LAL tests were performed on the final product in order to assure its safety for administration to experimental subjects.