Polymorphisms in TP53 (rs1042522), p16 (rs11515 and rs3088440) and NQO1 (rs1800566) Genes in Thai Cervical Cancer Patients with HPV 16 Infection

Abstract

The risk of cervical cancer development in women infected with HPV varies in relation to the individual host’sgenetic makeup. Many studies on polymorphisms as genetic factors have been aimed at analyzing associations withcervical cancer. In this study, single nucleotide polymorphisms (SNPs) in 3 genes were investigated in relation tocervical cancer progression in HPV16 infected women with lesions. Two thousand cervical specimens were typedby PCR sequencing methods for TP53 (rs1042522), p16 (rs11515 and rs3088440) and NQO1 (rs1800566). Ninetytwo HPV16 positive cases and thirty two normal cases were randomly selected. Analysis of TP53 (rs1042522)showed a significantly higher frequency in cancer samples (OR=1.22, 95%CI=1.004-1.481, p-value=0.016) whiledifferences in frequency were not significant within each group (p-value=0.070). The genotype distributions ofp16 (rs11515 and rs3088440) and NQO1 (rs1800566) did not show any significantly higher frequency in cancersamples (p-value=0.106, 0.675 and 0.132, respectively) or within each group (p-value=0.347, 0.939 and 0.111,respectively). The results indicated that the polymorphism in TP53 (rs1042522) might be associated with riskof cervical cancer development in HPV16 infected women. Further studies of possible mechanisms of influenceon cervical cancer development would be useful to manage HPV infected patients.