Screening Peptides Binding Specifically to Colorectal Cancer Cells from a Phage Random Peptide Library

Abstract

The aim of this study was to screen for polypeptides binding specifically to LoVo human colorectal cancercells using a phage-displayed peptide library as a targeting vector for colorectal cancer therapy. Human normalcolorectal mucous epithelial cells were applied as absorber cells for subtraction biopanning with a c7c phagedisplay peptide library. Positive phage clones were identified by enzyme-linked immunosorbent assay andimmunofluorescence detection; amino acid sequences were deduced by DNA sequencing. After 3 rounds ofscreening, 5 of 20 phage clones screened positive, showing specific binding to LoVo cells and a conserved RPMmotif. Specific peptides against colorectal cancer cells could be obtained from a phage display peptide libraryand may be used as potential vectors for targeting therapy for colorectal cancer.