WRAP53 Polymorphism, rs2287498: A Case Study in Northwest of Iran?

Abstract

<strong>Background:</strong> Non-coding RNAs apply regulations on expression or function of a gene. A class of non-coding RNAs, natural antisense transcripts, might overlap with their flanking genes and emerge a new complexity upon regulation. WRAP53, is a natural antisense transcript overlapped in a head-to-head manner on the opposite strand of TP53. It has 3 transcripts of which WRAP53β produces a protein and is needed for RNP biogenesis. Single nucleotide polymorphisms in this gene are associated with cancer susceptibility. <strong>Methods:</strong> In this study, we investigated the impact of WRAP53 Ex2+19 C>T polymorphism (rs2287498) in breast cancer susceptibility in Iranian-Azeri women, by tetra-primer amplification-refractory mutation system-polymerase chain reaction (tetra-ARMS PCR) method, in 222 patients women with breast cancer. We analyzed our data using javastat statistics package (http://statpages.org/ctab2x2.html) online software for allele and genotype frequencies and SPSS v.24 for evaluating rs2287498 association with clinicopathological features. Also, in silico experiments were carried out for predicting the second RNA structure with mfold v3.6, and for prediction of amino acid substitution effect with online software polyphen2. <strong>Results:</strong> Our results show a statistical significance of tumor size with the risk of breast cancer (p-value=0.036) but no significant genotype frequencies of rs2297498 and clinicopathological features with breast cancer susceptibility. in silico analysis estimated no significant changes in RNA or protein for this polymorphism. <strong>Conclusion:</strong> In conclusion, these findings suggest no relationship between rs2287498 and breast cancer susceptibility except with tumor size which confers a possible implication as a prognostic marker in relation to the size of the tumor.