Determination of electrophoretype of rotaviruses causative agents of diarrhea in children under two years oid referred to Tehran and Zahedan hospitals

Abstract

rotavirus infections are one of the etiological agents of infantile and children,s gastroenteritis.in this study 450 fecal samples from children under 2 years old from cities of Tehran nad Zahedan were collected.of these specimens.73 samples which were cosidered as moderately positive(++) by ELISA were selected.SDS-PAGE analysis showed fifty six different rotavirus electrophoretypes from which eight and forth eight patterns belonged to Zahedan and Tehran samples respectively.basically,from eight patterns of Zahedan samples,five(71.5%) were related to L and two (28.5%) to S rotavirus group A which includes seven different electrophoretypes.from forty eight patterns of Tehran samples,thirth six belonged to L series with twenty six defferent electrophoretypes (76.5) and twelve to S types with eight different electrophoretypes (23.5%).in addition, a mixed S and L electrophoretype and two abnormal genom of rotavirus with the pattern of 2&3&3&4 were identified.different electrophoretype of rotaviruses in Tehran and Zahedan showed a high diversity in comparison with electrophoretic patterns from other countries.