A survey on the influence of intra- peritoneal injection of hydrocortisone in the rate of intra abdominal adhesion bands in rats

Abstract

Occurrence of adhesions after pelvic and abdominal surgery is a well known problem. Previous surgical procedures are by far the most common cause of intra abdominal a adhesion. In over 10℅ of infertile women, adhesion formation is the main cause of their problem. Moreover postoperative adhesions are the most common cause of intestinal obstruction in the Western world. Although the cause of adhesion is unknown, but any trauma with mechanical, chemical, physical or infectious origin, to the peritoneum can cause a response in the peritoneal surface with subsequent adhesion formation. It is known that the basis of most studies, investigating the reduction of adhesion bands, are administration of anti- inflammatory and immunosupprestive drugs. In this study too, the intraperitoneal injection of hydrocortisone acetate for prevention of adhesions has been evaluated.fourteen rats with the mean weight of gr+_11.02 (X+_SD) were selected. After laparotomy and crashing the peritoneum covering uterus the peritoneal cavity was poured over by talc powder suspension which led to adhesion formation. Then animals were divided into two groups : the first group (n=7) received intraperitoneal injection of hydrocortisone acetate (25mg/kg) and the second group (n=7) received normal saline in the same volume. On the 28 th postoperative day, rats were killed and after relaparatomy they were evaluated with swolin system for adhesion formation. In the case group all the rats had grade II adhesion while in control group 6 rats had grade II and one rat had grade III adhesion. There was no significant difference in the rate of adhesion between two groups. Therefore according to this study, it can be concluded that intraperitoneal injection of hydrocortisone has no effect in decreased adhesion formation.