Role of denture-wearing on colonization and antifungal resistance of oral Candida albicans isolates in healthy people

Abstract

The primary focus of the present study was to evaluate the occurrence of Candida albicans isolates<br />in the oral cavity and its probable correlation with dental implant applications and prosthesis. We<br />collected oral swabs from patients who had attended private dentistry clinics, followed by stringent<br />and controlled antifungal susceptibility testing and calculation of colony forming units (CFUs).<br />Amphotericin B, Fluconazole and Itraconazole were three antifungals tested by CLSI M27-A2 broth<br />microdilution protocol. The MIC ranges for three tested antifungals in Candida albicans isolates<br />were obtained as 0.0625-1, 0.125-16 and 0.0313-0.5 μg/ml, respectively. Additionally, in non-<br />Candida albicans isolates, MIC ranges for three antifungals were achieved as 0.25-1, 0.125-16 and<br />0.0313-0.125 μg/ml, respectively. MIC50 values of both tested azoles in the Candida albicansgroup<br />were higher than related values in the non-Candida albicans group. Moreover, CFU counts for<br />denture-acquired people were higher than for participants not wearing denture applications,<br />indicating the proposal that the surface of dentures or any other synthetic implants in the oral cavity<br />may result in providing an appropriate environment for the colonization of yeasts.